earticle

영어

The optimum conditions for the production of tryptophan using a recombinant Klebsiella pnuemoniae phe A tyr A trp R/pSC 101-trp+ and its plasmid stability during tryptophan production were studied. The optimum temperature was 37℃ and the specific growth rate was 1.05h-1 at 37℃. Tryptophan production was increased by glucose fed-batch culture, and tryptophan was accumulated to 0.175 g/l after 36 hrs. This amount was about 1.2 and 1.6 times greater than that obtained from batch culture and flask culture, respectively. The stability of the strain in fed-batch cu1ture was greatly different from that in repeated flask culture. After 6 generation, 95% of total cells was stable in repeated flash culture, but in fed-batch culture only 50% was stable.

한국어

유전자 조작된 Klebsiella pnuemonia를 이용하여 트립토판 생산을 위한 최적 조건 및 플라스미드 안정성에 대한 연구를 수행하였으며, 최적온도는 37℃, 최적온도에서의 비증식속도는 1.05h-1로 측정되었다. 포도당을 기질로 사용하여 첨가회분배양 36시간 후의 트립토판 농도는 0.175g/l 이었으며, 이 결과는 다른 회분배양이나 플라스크 배양에 비해 1.2 및 1.6배 증가한 수치이다. 첨가회분배양에서의 균주안정성은 플라스크에서의 연속 교대 배양에서는 95%가 유지되었으나 첨가 회분배양에서는 50%만이 유지됨을 측정했다.