earticle

영어

The aims of this sutdy was to investgate the action mechanism of polycation on the B-glucan synthetase II (GS II) related to cell wall synthesis in suspension cultured carrot cells. In the suspension cultured cells treated with poly-L-Iysine(12uM) and poly-L-ornithine (12uM) having ploycationic nature, GS II activity increased about 40% and 50% than that of the control respectively. And similar response was observed when ATP and NaF were treated. On the other hand, ploy-L-lysine and ploy-L-ornithine did nor affect the membrane permeability. Phorbol-12-myrlstate-13-acetate (TPA), activator of protein klnase, increased about 35% and 1-(5-isoquinolinyl sulfonyl)-2-methyl-piperrazine (H-7), inhibitor of protein kinase, decreased about 30% of GSII activity than that of control. These results suggest that polycation plays a role in the cell wall synthesis by increasing GS II activity through phosphorylation

한국어

당근 현탁배양세포에서 세포벽 합성 효소인 GSII의 활성에 다가양이온의 작용기작을 조사하였다. 다가양이온의 특성을 가진 poly-L-Iysine과 poly-L-ornithine은 GSII의 활성을 40~50% 정도 촉진 시켰으며, ATP도 NaF도 이와 비슷한 결과를 나타냈다. 한편 poly-L-Iysine과 poly-L-ornithine은 막투과성에는 영향을 미치지 못하였으며, protein kinase의 활성제인 TPA는 GSII의 활성을 대조구에 비해 35%정도 증가시켰고 억제제인 H-7은 30%정도 감소시켰다, 이러한 결과들은 다가양이온이 인산화 과정을 통해 GSII의 활성을 증가시켜 세포벽의 합성을 증진시키리라 사료된다