earticle

영어

An optimal composition of medium for hyaluronic acid production and some characteristics of its from Streptococcus zooepidemicus were investigated. The hyaluronic acid from S. zooepidemicus was reached maximum level in the BY-medium containing 0.1% beef extract, 0.1% yeast extract, 3.0% glucose, 2.0% peptone, 0.1% NaCl and 0.5%CaCO3 (pH 7.5) at 37℃ for 36 hours with shaking. Addition of CaCO3 to the medium was necessary to neulralize the lowered pH which was resulted from hyaluronic acid production. Molecular weights of extracelluar and cellular hyaluronic acid produced by the strain were 1-1.4x106 and 5x106, respectively. The amount of extracellular hyaluronic acid was 91.9% of total hyaluronic acid produced and the vest was all intracellular.

한국어

Streptococcus zooepidemicus에 의한 hyaluronic acid 생성의 최적 배지 조성은 batch cultre 조건에서 0.1% 쇠고기 추출물, 0.1% 효모 추출물, 3.0% 포도당 2.0% peptone, 0.1% 식염 및 0.5% CaCO3 이였으며, 배지의 포기 pH는 7.5로서 37℃에서 36시간 진탕배양하는 것이 양호했다. 특히 공시균의 생육에 수반되어 hyaluronic acid가 생성되므로 배지의 pH를 중화하기위해 CaCO3의 첨가는 필수적이었다.