원문정보
Separation of Lipases Using Reversed Micelles
초록
영어
The liquid-liquid extraction of lipase A from Candide cylindracea and lipase B from porcine pancrease was carried out using reversed micellar organic solvents. Effects of various factors such as ionic strength, pH, and species and concentration of surfactant, on lipase solubilization were studied. A cationic surfactant cetyl-trimethyl ammonium bromide (CTAB) in isooctane/nhexanol(1:1) was found to be an effective solvent and its optimum concentration was 50 mM. KCl among various salts tested was the most effective and the efficiency of solubilization of lipase increased with decreasing the ionic strength of salts. The maximum activity and solubiliz ation of protein were obtained at pH 8. The stripping efficiency has a maximum value at pH 4 and increases with KCl concentration in the range of 0.2∼1.0 M. After the solubilization and stripping, the overall recovery efficiency of mass and specific activity of lipases was 62% and 66%, respectively.
목차
서론
실험재료 및 방법
효소용액 제조
추출용매와 역추출용액의 제조
실험 과정
단백질의 농도와 활성도 분석
역미셀의 수분함량의 조사
결과 및 고찰
단백질 추출
계면활성제의 영향
단백질 구조와 수용액 pH의 영향
이온감도와 염의 영향
단백질의 역추출
요약
참고문헌