원문정보
섬유소 가수분해반응에 관한 연구(I) -효소흡착에 대한 섬유소의 구조적 특성-
초록
영어
The structural properties of cellulose are significantly changed with the progress of hydrolysis reaction. The effects of changes on such properties of cellulosic substrate as crystallinity, amicessibility of enzyme to the active site of cellulose surface, and particle size on the kinetics of enzymatic hydrolysis have been studied. Among those physical studies, the apparent surface active site of cellulose particle was found to have the most significant effect on the hydrolysis kinetics. Based on the experimental results, the adsorption affinity of enzyme and hydrolysis rate were mainly influenced by the surface roughness of cellulose particle. The extent of accesssible active site may be expressed as the change of particle diameter. The Langmuir isotherm was proposed in terms of enzyme activity to explain the actual action of enzyme protein.
목차
INTRODUCTION
MATERIALS AND METHODS
Enzyme
Cellulosic Substrate
Determination of Structural Parameters
Adsorption Experiment
Particle Size Distribution Analysis
Analytical Methods
RESULTS AND DISCUSSION
Relationship between Adsorbed Soluble Protein and Enzyme Activity
Adsorption of Celluase Enzyme on Cellulose Effect of Structural Parameters on the Adsorption
Evaluation of apparent adsorption affinity on the cellulose surface
요약
NOMENCLATURE
GREEK LETTERS
REFERENCE