원문정보
Characteristics of B-glucosidase Immobilized on the Diazotized Chitin in Bioreactors
초록
영어
Diazotized chitin (CHITN) was synthesized reacting with NaN3 and HCl to alkaline hydrolyzed chitin for the support of immobilized enzyme. Immobilized B-glucosidase on diazotized chitin(CHITN-Gase) was produced reacting with glutaraldehyds as bifunctional reagent. CHITN-Gase activities were determined reacting with p-nitrophenol-B-D-glucopyranoside in plug flow reactor as a reference. Optimum temperature, optimum pH, reaction constant and deactivation rate were determined with variation of flow rate and H/D. The particle size of immobilized enzyme in the best was, 35 mesh (CHITN35-Gase). The optimum conditions of immobilized enzyme were in temperature and 5.0 in pH. Diameter and flow rate of plug flow reactor in the best was 8.5mm in diameter and 0.8ml/min in flow rate. Reaction constant was mainly influenced by electrostatic force. The best glucose hydrolizing activities of CHITN3 5-Gase was 3.3410-5 M/1. while that of native-B-glucosidase was 2.4410-5 M/1.
목차
서론
재료 및 방법
재료
효소
기질
완충용액
효소의 활성 측정
B-glucosidase의 고정
반응기
실험방법
결과 및 고찰
효소의 고정
온도 및 pH의 영향
Km' 및 Vmax'의 비교
활성 상호 관계
효소 불활성화 속도
요약
참고문헌