원문정보
Investigation of Brassinosteroid substances in the shoots of hybrid type korean rices
초록
영어
The production of bassinosteroid-like substances of two hybrid types of Korean rice, Jangseongbyeo, Taebackbyeo were investigated. The shoots at the maximum tillering stage were extracted and purified by solvent fractionation, silica gel adsorption chromatography Sephadex LH-20 chromatography, charcoal adsorption chromatography, Bondesil chromatography and HPLC of reverse phase, successively. Biological activities of each purification step were monitored by the rice lamina inclination test. Higher activities against the rice lamina inclination test in the each purification step showed that the shoots of two cultivars biosynthesize brassinosteroids. Two cultivars also showed a similar distribution of biological activities of endogenous brassinosteroids detected by HPLC
목차
서론
재료 및 방법
실험재료
추출 및 용매분획
Silica gel 흡착 chromatography
Sephadex LH-20 chromatography
Charcoal 흡착 chromatography
Bondesil chromatography
HPLC
생물검정법
결과
용매분획
Silica gel 흡착 chromatography
Sephadex LH-20 chromatography
Charcoal 흡착 chromatography
Sephadex LH-20 chromatography
Bondesil chromatography
C18 column을 사용한 reverse phase의 HPLC
ODS column을 사용한 reverse phase의 HPLC
고찰
요약
사사
참고문헌