원문정보
유전자 재조합 대장균을 사용한 Alpha-interferon의 생산과 분비;제4부. Ampicillin 및 Inducer의 Alpha-interferon의 생산과 Plasmid 안정성에 미치는 영향
초록
영어
We studied the production and excretion of alpha-interferon in recombinant Escherichia coli harboring plasmid pIF-III-B, which carries alpha-interferon gene under the control of lipoprotein and lacUV5 promoter, and lac operator. Basically, the effects of concentrations of ampicillin and an inducer, IPTG, for the expression of the cloned gene, on the productions of alpha-interferon and plasmid stability were studied. The highest production of alpha-interferon was observed at 50 mg/1 of ampicillin concentration and 0.5 mM of IPTG. The plasmid pIF-III-B was maintained very stably in medium with ampicillin but segregated rapidly in medium without ampicillin. Also, the plasmid was segregated more rapidly in medium with an inducer higher than 0.5 mM.
목차
INTRODUCTION
MATERIALS AND METHODS
Bacterial strains, plasmid and culture conditions
Determination of alpha-IFN activity
Preparation of samples for the assay of alpha-IFN
Determination of plasmid stability
RESULTS AND DISCUSSION
Effect of ampicilin concentration on the production of alpha-IFN
Effect of IPTG concentration on the production of aiPha-lFN
Plasmid stability
ABBREVIATIONS
요약
REFERENCES