원문정보
Selection of Protoplasts-Derived Cell Lines for High Yields Indole Alkaloids from Suspension Cultures of Vinca (Catharanthus roseus)
초록
영어
To produce economically important indole alkaloids by cell cultures, we selected protoplastsderived clones (protoclones) of vinca (Catharanthus roseus) for high yields of catharanthine and ajmalicine. Protoplasts were enzymatically isolated from suspension-cultured cells. The highest plating efficiency (1%) was obtained when protoplasts were plated at a density of 1105 protoplasts/ml in a culture medium solidified with 0.4% Seaplaque agarose. The growth rates of 40 protoclones subcultured on a solid medium varied over a wide range. Protoclone VPC-6, which had the highest growth rate, was observed to produce relatively high yields of catharanthine and ajmalicine when cultured in a liquid medium. Although the original cell line did not produce catharanthine at a detectable level by HPLC, protoclone VPC-10 produced it at a level of 5.9ug/g fresh weight of cells for 10 days of culture. Under the same conditions, protoclone VPC-15 produced ajmalicine at a level of 133.6ug/g, of which productivity was improved about ,3 times than that of the original cell line. The results indicate that differences in the growth rate and indole alkaloid yield among the protoclones reflect the somaclonal variation in suspnsion-cultured cells
목차
서론
재료 및 방법
갤러스 유도 및 현탁배양
원형질체 유리 및 배양
Plating efficiency의 측정
Protoclone의 생장률 측정
세포배양에 의한 indole alkaloid 생산
Indole alkaloid 추출 및 분석
결과
원형질체 배양
Plating efficiency에 미치는 원형질체 배양 밀도 및 gelling agent의 영향
Protoclone간의 생장률 변이
Protoclone간의 indole alkaloid 생산성 변이
고찰
요약
감사
참고문헌