원문정보
Effects of Glucose and Glutamine Concentrations on Hybridoma Growth ann Anti-HBsAg MAb Production in Enriched Medium
초록
영어
To improve the growth of mouse hybridoma 2c3.1 secreting anti-Hepatitis B surface antigen monoclonal antibody (anti-HBsAg MAb), we had constructed an enriched medium and observed the effects of fetal bovine serum and serum-free supplements including human serum albumin, 'insulin and transferrin', and monoethanolamine. For further enhancement of growth, the concentrations of two major energy sources, glucose and glutamine, were strengthened with various ratios in the enriched medium. Maximum cell growth and monoclonal antibody production obtained in various ratios of glucose/glutamine with an inoculation concentration of 2x105 cells/ml were 0.73x106-4.62x106 cells/ml and 65.1-422.6 ug/ml, respectively. Glutamine was round to be a major energy source and a limiting nutrient in comparison to glucose for 2c3.1 cell cultivation in enriched media with low serum.
목차
서론
재료 및 방법
시약 및 배지
Cell Line
농축배지의 제조와 세포배양
항체의 ELISA 분석
기타 분석
결과 및 고찰
농축배지에서 초기 Glucose와 Glutamine 농도가 2c3.1 세포 증식에 미치는 영향
Glucose와 Clutamine의 초기 농도에 따른 Glucose 와 Glutamine의 소비 비교
단일클론항체 생산에 미치는 Glucose와 Glutamine의 초기 농도의 영향
요약
참고문헌