원문정보
Effects of Nutrients and Cell Aggregate Size on the Biosynthesis of Carotenoid in Daucus carota Suspension Culture
초록
영어
The effects of nutrients and cell aggregate size on the cell growth of D. carota and the biosynthesi of carotenoid were investigated. Highest carotenoid content was obtained with sucrose as a carbon source and the equal ratio of ammonium to nitrate. High phosphate concentration stimulated the carotenoid biosynthesis in D. corota. 2,4-D inhibited the cell growth but stimulated the specific carotenoid content at high concentration. By modifying the medium composition based on these findings, three times higher specific carotenoid content and 2.5 times higher total carotenoid content were obtained as compared with the results obtained with basic MS media. Biosynthesis of carotenoid was found to be affected by cell aggregate size; high carotenoid production was obtained from the large aggregated cells resulted from high sucrose concentration.
목차
서론
재료 및 방법
세포주 및 배지 조성
현탁 배양
세포 무게 측정
catotenoid 분석
당농도 분석
결과 및 고찰
식물세포의 성장과 carotenoid 생활성에 미치는 배지 조성의 영향
세포 응집 크기에 따른 carotenoid 생합성 효과
요약
감사의 글
참고문헌