원문정보
Production of Rabbit Anti-Bovine Heart Pyruvate Dehydrogenase Antibody
초록
영어
Rabbit anti-bovine heart PDH antiserum was raised against El(a, b) isolated from PDC, and then applied to detect Ela and Elb. Appropriate amounis of El were fractionated by SDS-PAGE and electrophoretically transferred to nitrocellulose membrane. The Ela and Elb on the membrane were incubated with anti-El antiserum and identified by GAR-HRP system. It has been found that the immunodetection sensitivity of Ela and Elb were directly proportional to the amount of antigen and transfer time. The lengthy transfer times increased the immunodetection sensitivity of Ela and Elb. The maximal detection sensitivity of Western blotting of Ela and Elb was achieved at 3.5 V/cm for 16-hour transfer under these experimental conditions.
목차
서론
재료 및 방법
피루브산 탈수소효소(E1a, b)의 분리
항 피루브산 탈수소 효소(E1a,b)의 항혈청 생산
E1a와 E1b의 Western Blotting
결과 및 고찰
피루브산 탈수소 효소 단백질(E1a,b)의 분리
피루브산 탈수소 효소 항체 생산
PDH의 Western Transfer
요약
감사
참고문헌