생물반응기에서 형질전환된 식물조직 배양을 위한 기초연구


Basic Studies on Cultivation of Transformed Plant Tissue in Bioreactor

박성화, 박돈희, 김재형, 허병기, 조영일

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Growth properties of carrot hairy roots transformed by Agrobacterium rhizogenes were compared in flask and bioreactor. Oxygen transfer coefficient KLa was measured during the cultivation in bioreactor. In flask cultures, initially sucrose 30g/l was nearly exhausted after 20days. pH was dropped from initially 5.8 to 4.79 after 4 days, but it is stable after that time. Finally, after 28 days, hairy roots were grown about twelve times. In view of the results studied optimum conditions, hairy roots were maintained high growth rates in sucrose 50g/l, pH 5.8, total nitrogen 60mM. Also in bioreactor cultures, fixed stainless sieve in bottom and aerated 0.31 vvm, the results of cultivation by the use of sucrose 50g/l had grown about twenty-eight times and pH variations were liked in flask. As a results, growth rate of 1.756g fresh weight/day/g inoculum in bioreactor were higher about three times than 0.57g fresh weight/day/g inoculum in flask culture. KLa values were showed a tendency to decrease from 0.209 min-1 to 0.068 min-1.


 재료 및 방법
  hairy roots 유도
  회분식 배양
  생물반응기 배양
  생체량 측정
  총당 및 환원당 분석
  Anthocyanin 분석
  물질전달계수(KLa) 측정
  Gassing-in liquid법에 의한 전달계수 측정
  Dynamic법에 의한 전달계수 측정
 결과 및 고찰
  회분식 배양결과
  탄소원의 영향
  질소원 및 pH 영향
  물질전달계수(KLa) 측정결과
  생물반응기 배양결과


  • 박성화 Sung-Hwa Park. 전남대학교 화학공학과
  • 박돈희 Don-Hee Park. 전남대학교 화학공학과
  • 김재형 Jae-Hyung Kim. 전남대학교 화학공학과
  • 허병기 Byung-Ki Hur. 인하대학교 생물공학과
  • 조영일 Yung-II Joe. 연세대학교 화학공학과


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