원문정보
Study on Steroid Acly Transferase in the Rat Brain
초록
영어
The characteristics of steroid acyl transferase were studied in the rat brain with (4-14C)-dehydroepiandrosterone(DHEA). The results could be summarized as followings: The enzyme system responsible for the biosynthesis was localized at the microsome fraction. The optimum pH of this enzyme was 4.6 When DHEA was utilized as substrate, 5-pregnenolone was proved to be a competitive inhibitor. However testosterone was a noncompetitive inhibitor. The acylation at 3B-hydroxyl group was favored when the hydrophilicity at Cl7 position increased. However, this acylation at C3 was very low when A ring was aromatic. The acylation at Cl7 hydroxyl group reguired an absolute 17B-conformation.
목차
서론
재료 및 방법
Subcellular Fraction의 제조
반응 조건
Steroid의 추출 및 분리
동정 및 정량
결과 및 고찰
생합성된 Steroid-acyl 유도체의 동정
Steroid Acyl 전이효소의 분포
Steroid Acyl 전이효소의 일반적 특성
업체 특이성
결론
감사
참고문헌