원문정보
유전자 재조합 대장균을 사용한 Alpha-interferon의 생산과 분비: 제2부. 재조합 균주의 생장특성
초록
영어
The growth behavior of recombinant Escherichia coli cells having plasmid pIF-III-B, which carries human alpha-interferon gene under the control of lpp promoter, lac promoter and lac operator, was studied by using of various E. coli host strains. Expression of the alpha-IFN gene is controllable by using inducer IPTG because the plasmid also contains lacI gene which produces lac regressors. The repressors block the transcription of alpha-IFN gene. There were considerable differences in cell growth according to the host strains used. Cell growth was inhibited not only by plasmid pIF-III-B itself but also by the induction of alph-a-IFN gene expression. Growth inhibition caused by the plasmid itself was more serious than that caused by the induction of alpha-IFN gene expression.
한국어
대장균의 lipoprotein promoter, lactose promotor 및 operator 와 lipoprotein의 signal sequence 를 가지는 vector에alpha-IFN 유전자가 cloning된 plasmid pIF-Ill-B를 여러종류의 대장균 숙주 세포에 형칠전환하여 alpha-IFN 의 생산성, 생장특성을 조사하였다. 또한 plasmid 자체와 cloning된 alpha-IFN 유전자의 발현유도가 세포생장에 미치는 영 향을 조사하였다.
목차
INTRODUCTION
MATERIALS AND METHODS
Bacterial strains and Plasmid and culture conditions
Induction of gene expression
Transformations
Preparation of recombinant cell extract for the assay of alpha-lFN
Determination of alpha-lFN activity
RESULTS
Growth behavior of various E. coli strains harboring Plasmids
Cloned alpha-lFN gene Product formation
Effect of Plasmid PIF- III-B and induction of gone expression on cell growth
DISCUSSION
ABBREVIATIONS
요약
REFERENCES