원문정보
유전자 재조합 세포 발효의 온.라인 유도 : 제 3부. 조건 최적화 및 콤퓨터 자동 제어
피인용수 : 0건 (자료제공 : 네이버학술정보)
초록
영어
The computer interfaced by the necessary hardwares and the software developed in PARTs I and II were used to control the fermentation with recombinant cells in an on-line feedback-feed-forward manner. The recombinant cells were induced for expression either thermally or chemically. Very accurate controls with fast response and no oscillation could be performed.
한국어
필드 하드웨어와 소프트웨어를 자체 개발하고 컴퓨터 인터페이싱을 수행하여 유전 재조합세포 발효 공정을 온.라인으로 휘드.백 및 휘드.포워드 식 자동제어 할 수 있었다. 유전자 재조합 세포들이 발현을 위하여 온도 유도 및 화학약품 유도 방법을 사용하였다. 빠르고 안정된 반응을 보이고 정확한 자동 제어를 수행할 수 있었다.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Bacterial Strains
Plasmid
Plasmid DNA Isolation and Agarose Gel Electrophoresis
Expression of NS1 gene
Purification of NS1 Protein and its Analysis
Culture Conditions
Determination of Induction Point
Determination of Plasmid Stability
Measurement of Dry Cell Weight
RESULTS AND DISCUSSION
Plasmid Stability
Effects of Induction Temperature
Effects of Switching Time
Effects of Harvesting Time
CONCLUSION
요약
ACKNOWLEDGMENT
REFERENCES
INTRODUCTION
MATERIALS AND METHODS
Bacterial Strains
Plasmid
Plasmid DNA Isolation and Agarose Gel Electrophoresis
Expression of NS1 gene
Purification of NS1 Protein and its Analysis
Culture Conditions
Determination of Induction Point
Determination of Plasmid Stability
Measurement of Dry Cell Weight
RESULTS AND DISCUSSION
Plasmid Stability
Effects of Induction Temperature
Effects of Switching Time
Effects of Harvesting Time
CONCLUSION
요약
ACKNOWLEDGMENT
REFERENCES
저자정보
참고문헌
자료제공 : 네이버학술정보