earticle

영어

The feasibility of using bacterial cultures with the ultimate aim for the marine environmental clean-up was explored. The present study reports on the bacterial elimination of nitrogens and phosphorus by strains CK-10 and CK-13 isolated from shrimp farming pond. The strains were identified as genus Bacillus on the basis of BIOLOG test, and designated as Bacillus sp. CK-10 and Bacillus sp. CK-13, respectively. Removal of nitrogens (NH4 +, NO2 -, or NO3-) or phosphorus (PO4 -3) as single N or P source was studied with single cultures under aerobic conditions. Complete elimination of all nitrogens in the concentration range of 100-400 μM was achieved in single cultures as well as co-cultures within the given incubation period. Similar results were obtained from the test cultures containing 125-599 μM PO4 3-. Simultaneous removal of all N/P was monitored in the co-cultures. As the results, 400 μM NH4 + and NO2
- were eliminated within 12 hrs and 400μM NO3 - and 500 μM PO4 -3 were completely disappeared within 36 hrs from the media. The work demonstrated that co-cultures improved the concurrent removal of N/P from the media.