원문정보
Label-free Detection of Biomolecular Specific Interaction by Optical Biosensors
초록
영어
Label-free optical methods for the monitoring of interactions between biological molecules have become increasingly popular within the last decade. A rising number of publications have demonstrated the benefits of direct biomolecular interaction analysis(BIA) for biology and biochemistry, such as antigen-antibody interactions, receptor-ligand interactions, protein-DNA, DNA- intercalator, and DNA-DNA interactions. This article gives an overview of the historical development, principle and application of label-free optical biosensor to examine the functional characteristics of biospecific interaction, such as kinetics, affinity, and binding position of biomolecular between an immobilized species at the transducer surface and its dissolved binding partner.
목차
서론
원리와 방법
고정화(Immobilization)
기초적인 변환기 표면변형(modification)과 기능화(functionalization)
비특이성 결합을 최소화하기 위한 변환기 표면변형
비표지 생체물질의 고정화(Immobilization)
변환기(Transducers)
분석절차/자료처리(평가)
직접측정
간접 검출법
결론
REFERENCES