earticle

영어

Lactobacilli have been considered to play important roles in the health of human vagina. They secrete inhibitory substances to prevent vaginal infection by pathogenic organisms. In a previous study, we have isolated several lactobacilli from Korean woman and one of them (KLB46) was selected and indentified as Lactobacillus crispatus which showed high antimicrobial activity. In this study, cold adaptation prior to subsequent stresses exposure was examined whether L.
crispatus KLB46 maintain the viability better than the non-adapted cells under stresses. For pharmaceutical formulation, the lyophilization process is required where stresses such as freezing/thawing and dehydration are routinely applied. Formulated L. crispatus KLB46 can be used for ecological treatment of bacterial vaginosis. The response of cold adapted cells to other
environmental stresses such as acid, heat, ethanol, NaCl, and H2O2 was also examined. The results showed that coldadapted cells maintained higher survival rate compared with the non-adapted cells (freezing-thawing, 3-folds; dehydration; 3-folds; acid, 3-folds; heat, 10-folds). However, we did not observe any positive effect of cold adaptation on other stresses such as ethanol, NaCl and H2O2. When chloramphenicol was added during cold adaptation, adaptation effect was abolished. This confirms that de novo protein synthesis is necessary during the adaptation process. Moreover, we have identified cold shock protein homolog that codes for a major cold shock protein by PCR amplification using degenerate primers.

한국어

본 연구에서는 L. crispatus KLB46을 저온 전처리함으로서 제제화 과정에 받게 되는 얼림과 녹임, 건조 스트레스뿐만 아니라, 여러 다른 환경 스트레스에 대한 내성이 증진된다는 것을 확인하였다. 그리고 내성 증진에 신규 단백질 합성이 필요함을 확인하였으며 나아가, 저온 충격 유전자(csp)를 확인하였다. 따
라서 이 균주를 제제화 하기 위한 방법으로 저온 전처리를 이용할 경우 생균력 유지에 큰 효과를 얻을 수 있다고 사료된다.