earticle

영어

Genetic transformation in dandelion (Taraxacum mongolicum Hand.) was studied. We used for transformation by Agrobacterium tumefaciens strian LBA4404 harboring a binary vector pBI121 carrying the CaMV 35S promoter-GUS gene fusion used as a reporter gene and NOS promoter-NPTⅡ gene as a positive selection marker. To obtain transformed plants, leaf explants of dandelion were cocultured with Agrobacterium tumefaciens LBA4404 for 10 mins, then transferred to MS medium containing 1 μM IAA, 1 μM BA, 100 μg/mL carbenicillin and 50 μg/mL kanamycin
sulfate. After two weeks of subculture of the explants, kanamycin-resistant shoots were formed on explants survived. When subjected to GUS histochemical assay, all of the regenerants showed the GUS-positive responses. Plantlets were be transformed to soil for further growth.

한국어

국화과에 속하는 다년생 초본식물인 민들레가 Agrobacteria에 대한 숙주로서의 가능성을 조사하고 여러 가지 유용한 유전자를 민들레로 도입시키기 위해, 민들레잎 절편을 pBI121으로 형질전환된 Agrobacterium tumefaciens LBA4404와 10분 동안 공동배양하여 형질전환시킨 후, 1 μM IAA, 1 μM BA, 50 μg/mL Km과 100 μg/mL Cb이 함유된 MS 배지에서 약 2주 후에 multiple shoot를 유기시켰다. 유기된 shoot로부터 유식물체를 얻었으며, 형질전환을 확인하기 위해 GUS활성을 측정한 결과 양성 반응을 보였다.