earticle

영어

The removal of nitrogen compounds from waste water is essential and is often accomplished by biological processes. The denitrifying bacterium, Paracoccus denitrificans (KCTC 2530), was employed to study the characteristics and the denitrification differences of Permeabilized strains and untreated strains. The permeabilization rate increased with increasing toluene concentration, but some part of the toluene contributed to denaturing the detachment of proteins from the plasma membrane. Permeabilized Paracoccus denitrificans had long lag phase and high specific growth rate in cultivation, and showed excellent denitrification characteristic compared with untreated strains. But, in both cases, the denitrification ability was significantly reduced after 4 or 5 denitrifications. It seems that the strains fall into the death phase when the nutrient was exhausted. When the nutrient recovered to its initial level, the denitrification ability also
recovered to the normal level. The results obtained were encouraging enough to apply to practical water treatment situation.

한국어

탈질화 균주인 P. denitrificans를 유기용매인 톨루엔으로 permeabilization 처리하는데, 톨루엔의 농도와 처리시간에 따른 영향을 관찰한 결과 톨루엔의 농도에 비례하여 permeabilization 처리는 잘 이루어지지만 일정농도 이상의 톨루엔 농도에서는 세포벽으로부터 탈리된 단백질의 denaturation에 톨루엔이 사용됨을 확인하였다. Permeabilization 처리된 P.denitrificans는 미처리 균주보다 우수한 탈질능력을 보였고, 반복탈질 처리 시에는 처리균주와 미처리균주 공히 초기 탈질처리 시간보다 짧은 시간 내에 같은 농도의 질산성 질소를 처리하는 경향을 보였다. 3-4회의 반복탈질처리 후 질산성질소 농도와 영양물질을 초기농도로 회복시켜주었을 경우, 같은 속도와 경향으로 탈질처리 하는 능력을 보였다.