earticle

영어

A halotolerant and extracellular protease-producing yeast was isolated from traditional Meju and identified as a strain of Hansenular polymorpha by investigating its microbiological characteristics. The optimum pH, temperature and NaCl concentration required for the growth of Hansenular polymorpha S-9 were found to be pH 6.0, 30℃ and 0.5 M, respectively. Extracellular protease was produced maximally at 10 U mL-1 when Hansenular polymorpha S-9 was grown
on the medium containing 1.0% beef extract and 0.1 M NaCl for 12 hr at 30℃. About 13% of the angiotensinconverting enzyme (ACE) inhibitory activity was shown in the hydrolysates which were obtained from the digestion of soybean protein (6 mg) for 6 hr at 30℃ by the crude enzyme (1 U).

한국어

내염성이며 세포외 protease를 강력하게 생산하는 효모를 재래식 메주효모들과 자연계에서 분리된 내염성 효모들 가운데서 선별하여 Hansenula polymorpha로 동정하였다. Hansenula polymorpha S-9은 30℃, pH 6.0, 0.5 M NaCl을 함유한 YM배지에서 잘 생육하였고 이 균을 1.0% beef extract와 0.1 M
NaCl를 함유한 Beef extract 배지에 접종하여 30℃로 12시간 배양하였을 때 가장 많은 mL당 10 U의 효소가 생산되었다. 이와 같이 생산된 Protease(1U)를 대두 단백질(6 mg)에 첨가하여 30℃ 에서 6시간 가수분해시킨 후 얻은 생성물에서 약 13%의 angiotensin전환효소(ACE) 저해활성을 보였다.