원문정보
Improved Technologies to Produce Heterologous Proteins in Recombinant Escherichia coli.
초록
영어
Escherichia coli has been used as an expression work horse for foreign genes. This article summarized recent development in genetic engineering techniques for overproduction of medical proteins and industrial enzymes. Special emphasis was placed upon research activities concerning folding and refolding of inclusion bodies at genetic and fermentation levels. Plasmid and mRNA stabilization, development of strong inducible promoters, modification of
translational elements and reduction of proteolytic degradation were carried out to elevate an expression level of a target protein. Optimization of culture conditions, improvement of denaturation and renaturation steps and coexpression of molecular chaperones or foldase were accomplished to produce active proteins in soluble form. Fusion protein systems with selective separation and surface display technology were also performed in an effort to make the E. coli
expression system more effective and versatile.
목차
서론
생산성 향상을 위한 분자 생물학적, 발효 공학적 접근
재조합 대장균에서의 활성형 단백질의 생산
결론
REFERENCES