earticle

영어

The optimum conditions for the production of an antifungal antibiotic from Bacillus sp. YJ-63 were investigated. The optimumized medium consisted of 1.5% soluble starch, 1% tryptone and 0.5% yeast extract, and temperature and initial medium pH for production were optimal at 35℃ and pH 6.0, respectively. Production yield was significantly improved by shaking culture using 50 ml medium in 500 ml flasks. Under these conditions, the production of the antifungal antibiotic was growth-dependent, from 35 hrs into cultivation to the stationary phase and endospore formation

한국어

내열성의 항곰팡이성 항생물질을 생산하는 Bacillus sp. YJ-63으로부터 항생물질을 대량으로 생산하기 위한 최적조건을 검토한 결과는 다음과 같다.
항생물질을 생산하기 위한 배양조건은 35℃, pH 6.0이었으며, 최적생육 배지는 1.5% soluble starch, 1% tryptone, 0.5% yeast extract로 조성하여 500 ml의 삼각플라스크에 50 ml씩 분주하여 진탕배양하였다. 이와 같은 최적조건에서 항생물질은 정지기부터 생산되는 비증식연관형으로서 포자형성 이후부터 생산되었다.