earticle

영어

The biosynthesis of lovastatin, a cholesterol lowering agent formed by the filamentous fungus Aspergillus terreus, was examined in a 2.5 L jar fermenter. In batch bioreactor cultures conducted at various agitation rates, 400 rpm showed the best result in terms of lovastatin production. Notably, the effect of pH on lovastatin biosynthesis was found to be significant: when the pH was controlled at around 5.8 during the whole fermentation period, lovastatin concentration
reached 598 mg/L, which is much higher than the amounts obtained by pH-uncontrolled and pH 7.4-controlled fermentations. In addition, both L-histidine and L-tryptophan were observed to be favorable amino acids for the enhancement of lovastatin production when 6 g/L of the respective amino acids were supplemented at the beginning of the fermentation period. By further optimization of the production media and the physical environment, lovastatin production was increased to 836 mg/L (3.5 mg/L/hr) which is approximately 10 times higher than the productivity of the basic control culture.

한국어

고지혈증 치료제인 lovastatin을 Aspergillus terreus로부터 생산하기 위해 발효조 배양실험에서 교반속도와 pH에 대한 영향을 조사하였다. 최적 교반속도는 400 rpm이었고 pH는 5.8로 유지하였을 때 lovastatin 생산이 최대였으며, 교반속도보다 pH가 lovastatin 생산에 더 많은 영향을 미치는 것으로 나타났다. L-tryptophan과 L-histidine의 첨가시기에 따른 lovastatin 생산량을 실험한 결과, 둘다 발효초기부터 첨가하여 배양하는 것이 효과적이었다. L-tryptophan을 발효초기에 첨가한 최적배지와 최적 환경조건인 교반속도 400 rpm, pH 5.8에서 회분식 배양을 수행한 결과 기본배지를 이용하여 실험한 배양
결과보다 약 10배 정도 많은 836 mg/L이었고, 생산성은 3.5 mg/L․hr였다.