earticle

영어

An on-line glucose flow injection analysis system was developed and used for the automatic analysis and addition of glucose in the cultivation of a Saccharomyces cerevisiae in a korean paper digestion wastewater in order to increase the cell concentration. The system was composed of a ceramic sampler, a sampling valve, an injection valve, an immobilized glucose oxidase column, a debubbler, a flow cell with platinum electrodes, a potentiostat, a computer and
interface system, and tubing pumps. The glucose concentration of the wastewater medium was maintained at the low concentration of 176±31mg/l with the on-line FIA system, and by adding glucose and (NH4)2SO4, the cell concentration as total cell count can be increased by 3.1times.

한국어

한지자숙폐액을 이용한 Saccharomyces cerevisiae의 배양에서 glucose의 자동분석 및 첨가에 의해 균농도를 증가시킴으로써 폐액의 재이용성을 향상시키고자 하였다. 배양액의 glucose를 자동분석하고 자동첨가하기 위하여 ceramic sampler, sampling valve, injection valve, glucose oxidase column, debubbler, flow cell과 백금전극, potentiostat, computer와 interface card, 및 tubing pump들로 구성된 glucose 자동분석 및 첨가시스템을 제작하였으며, glucose의 자동분석에는 glucose oxidase를
이용한 on-line FIA법을 채택하였다. 효모의 배양중 glucose자동분석 및 첨가시스템을 사용하여 glucose를 자동첨가한 결과 배양액의 포도당농도가 176±31 mg/L 의 낮은 농도로 제어되었으며, glucose와 (NH4)2SO4의 첨가에 의해 총균수가 3.1배 증가하였다.