earticle

영어

A Saccharomyces cerevisiae isolated from a feed additive yeast product was cultivated in a Korean paper digestion wastewater in order to investigate the possibility of using it as substrate for the yeast. The yeast couldn't grow in the wastewater. It could grow in the wastewater diluted and the optimum dilution rate was 7.5. In batch cultivation with the jar fermenter the maximum total cell count was 1.34×107/mL and the maximum viable cell count was 1.1×107/mL. The
deigestion wastewater undiluted was added during the cultivation of the yeast in the diluted wastewater media in order to increase cell density. The highest total cell count of 8.2×107/mL was obtained by the addition of undiluted digestion wastewater. By adding (NH4)2SO4 and KH2PO4 together with the undiluted wastewater, the maximum cell concentration could
be obtained faster.

한국어

한지자숙폐액을 사료첨가효모의 생산기질로 재이용할 수 있는 가를 검토하기 위하여 사료첨가효모제품으로부터 분리한 Saccharomyces cerevisiae종을 한지자숙폐액에 배양하였다. 자숙폐액원액에서는 균이 증식하지 못하였으나, 희석액에서는 증식하였으며, 증류수로 7.5배 희석시 최대증식을 보였다. Jar fermenter배양에서의 최대총균수는 1.34×107/mL이었으며, 최대생균수는 1.1×107/mL이었다. 배양액의 균체농도를 증가시키기 위하여 배양중에 자숙폐액원액을 첨가하여 총균수를 최대 8.2×107/mL까지 증가시킬 수 있었다. 자숙폐액원액과 함께 (NH4)2SO4 및 KH2PO4까지 첨가함으로써 최대균체농도에 도달하는 시간을 단축시킬수 있었다.