earticle

영어

Protease-catalyzed liberation of sialyloligosaccharide from raw egg yolk was investigated in biphasic system, water-immiscible hexane system. Biphasic system Ⅰ, in which water was the continuous phase, was better than the opposite biphasic system Ⅱ in sialyloligosaccharide liberation. The optimal conditions of temperature, water content and reaction time were 30℃, 20%
and 12 hours, respectively. Protease activity was strongly influenced by the amount of water present in the reaction mixture. The liberation of sialyloligosaccharide was accelerated by protease pre-treatment at 30℃ in 0.2 M NaCl solution, prior to addition of hexane (Biphasic system

한국어

생난황으로부터 시알산 함유 올리고당을 직접 생산하기 위한 biphasic system(water-immiscible hexane system)의 최적 조건을 조사하였다. Biphasic system Ⅰ은 Ⅱ보다 생산수율이 높았으며,
온도, 함수율 및 반응 시간의 최적조건은 각각 30℃, 20%, 12시간이었다. Biphasic system Ⅰ에서, 난황을 가수분해하기 위한 3종류의 효소(protease A, corolase 7089, neutrase 0.5L)의 활성은 함수율과 온도에 강하게 영향을 받았으나, biphasic system Ⅱ에서는 온도(30℃와 50℃)의 영향을 거의 받지 않았다. 두 종류의 biphasic system에서 반응시간 12시간까지는 시알산 함유 올리고당의 생산량이 증가되었으나, 15시간 이후에는 거의 증가하지 않았다.