원문정보
초록
영어
To investigate the possible association between −2510A/G promoter polymorphism (rs1024611) ofchemokine (C-C motif) ligand 2 (CCL2) gene and rheumatoid arthritis in Korean population. DNAs were obtained from192 Korean rheumatoid arthritis patients and 270 healthy controls. CCL2 genomic variants (−2510A/G polymorphism)were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The −2510A/Gpromoter polymorphism was not associated with rheumatoid arthritis [genotype frequency (P=0.710); allele frequency(P=0.813, OR=1.033, 95% CI=0.79−1.36)]. The −2510A/G promoter polymorphism is not associated withrheumatoid arthritis, suggesting −2510A/G promoter polymorphism is not a susceptibility factor in the development ofrheumatoid arthritis in Korean population. However, G allele is a major allele in Asian including Korean, whereas amajor allele in Caucasian and European is A. The ethnic difference is present in −2510A/G promoter polymorphism.
목차
1. Introduction
2. Materials and methods
2.1 Study population
2.2 Extraction of DNA from blood
2.3 Screening of SNPs in area of CCL2 gene
2.4 Polymerase chain reaction (PCR) andrestriction fragment length polymorphism (RFLP)
2.5 Statistics
3. Results and discussion
References