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세포농도 연속 측정 및 제어용 광섬유 센서의 초기적 연구

원문정보

An Initial Study on the Optical Fiber Sensor for Continuous Monitoring and Control of Cell Mass

장일선, 정재칠, 김태진

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초록

영어

The randomly mixed type optical fiber sensor and the centralized type one were developed for the on-line, continuous, real time measurement and control of cell mass, which is one of the most important parameters in thebiochemical process. The whole system together with electronic measuring system were tested in the various strains andsolutions for the determination of the linearity of optical density OD and cell mass, and the stability. Also, the stabilityand linearity of the centralized sensor was better than those of the randomly mixed type sensor in the solutions of analogof culture medium such as rice wine, milk and yoghurt. But, the stability and the intensity were severely affected bythe contact part geometry of quartz window and fiber, the evenness of the window surface and the angle of thetransmittance and back scattering at the window. The measuring system was designed for the 4-20 mA output similarto that of commercially available Aquasant type of Mettler-Toledo. It can be concluded that the presently developedoptical sensor system can be extended its application to the biochemical process as long as its corresponding softwarefor individual strains are developed in advance.

목차

ABSTRACT
 1. 서 론
 2. 실험 방법
  2.1 광섬유센서의 제작
  2.2 광로간격 개발
  2.3 실험실적 안정성 평가
 3. 결과 및 고찰
  3.1 광섬유센서
  3.2 광섬유센서의 Cell Mass 농도의 변화에 따른 출력전압의 변화
  3.2 광섬유센서의 발효온도와 교반속도에 따른출력전압의 변화
 4. 결 론
 감사의 글
 References

저자정보

  • 장일선 Il-Sun Jang. 수원대학교 공과대학 화공생명공학과(Department of Biochemical Engineering, The University of Suwon)
  • 정재칠 Jae-Chil Jung. 수원대학교 공과대학 화공생명공학과(Department of Biochemical Engineering, The University of Suwon)
  • 김태진 Tai-Jin Kim. 수원대학교 공과대학 화공생명공학과(Department of Biochemical Engineering, The University of Suwon)

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