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Purpose: The purpose of this study was to identify the relation of Job Stress, Heart Rate Variability, Blood Pressure and Lifestyle on White-Collar Workers. Method: Data were collected from August to December in 2007. HRV was measured using Freeze-Framer. WSRI and Lifestyle data were collected from the self-reported questionnaire. Result: 1) Total score of WSRI was 32.88, H score was 47.92, L score was 16.90, systolic blood pressure was 138.88mmHg, diastolic blood pressure was 81.20 mmHg. 2) WSRI correlated with L score of HRV, BP, smoking, drinking positively. WSRI correlated with H score, exercise times negatively. H correlated with exercise positively and had negative correlation with BP and L. Systolic pressure correlated with smoking and drinking positively. Conclusion: WSRI, HRV, BP and lifestyle are interrelated strongly and they need to be monitor continually. It is considered that education of health and intervention with HRV make white-collar workers concern more about their health and care.