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Purpose: The purpose of this study were to investigate the health status and to identify the influencing factors on health status by gender among service workers. Method: Data were collected from the self-reported survey of 781 service workers in sales, food & lodging industry. The data were analyzed by descriptive statistics and multiple linear regression analysis using SPSS Win 10.0. Result: The influencing factors of male workers' health status were job satisfaction, standing hour during work time, physical work environment, job demand and family support. The influencing factors of female workers' health status were job satisfaction, emotional labor, physical work environment, regular exercise, standing hour during work time, social support and age. Conclusion: Gender specific occupational Health program for service workers should be developed in consideration of these influencing factors.