원문정보
초록
영어
Assisted reproductive technologies have been a critical therapy for human infertility and producing genetically superior livestock. Especially, in vitro cultivation (IVC) has become an important pipeline for realizing in vitro production since it is one of the most inefficient and undeveloped systems. Conventional method was focused by chemical approach to improve in vitro development of embryos. However, it had rather limitation in terms of low cell cleavage rate and blastocyst development. Recently, several trials were performed to mimic in vivo microenvironments; one is a glass oviduct culture system fabricated by glass microcapillaries1 and other representative is a microfluidic culture system.2 To mimic more close oviduct environment, we have constructed a oviduct-inspired in vitro cultivation system using
microfluidics. The device was fabricated by photolithography and PDMS replica molding process. The constriction parts in a IVC channel were contributed to mimic of peristaltic motion and the end of isthmus. Normal channel width was 200 ㎛ and the constriction parts were 140 ㎛, 150 ㎛ and 160 ㎛, respectively. The cleavage rate of in vitro fertilized eggs in the
system was almost 100% which was comparable to 88% in conventional microdrop method. Consequently, this platform would be helpful to increase the efficiency and quality of IVC technology.