원문정보
초록
영어
The homocysteine thiolactone, a reactive metabolic compound of homocysteine, has been implicated in human cardiovascular diseases. The increasing of total plasma homocysteine concentration lead to homocystinuria, which may cause to elevate the concentration of homocysteine thiolactone in the blood. It might be one of the risk factor which is responsible for the development of several vascular diseases. The present study is to demonstrate the harmful effect of homocysteine-thiolactone induced on calf pulmonary artery endothelial (CPAE) cells. CPAE were cultured in RPMI1640 medium supplemented with 10% FBS. CPAE were grown in humidified atmosphere of 5% CO2 in air at 37℃. Cells cultured with homocysteine thiolactone at concentrations ranging from 0.1mM, 1mM, 5mM and 10mM (dissolved in dH2O). After incubation, cells were stained with MTT. Cell viability was evaluated under the UV-visible spectroscopy at 540nm. By increasing the concentration of homocysteine thiolactone cells viability was significantly decreased. Our data provide the evidence to understand the effect of homocysteine thiolactone on the cardiovascular system. [Supported by grant No. R01-2006-000-10232-0 from MOST]