원문정보
초록
영어
Assuming that mental stress can be substituted to the electrical stress, we established a model of a in vitro electrical stress ES which can be compared with in vivo mental stress model. The effect of electrical stimulation was investigated in the culture of human leukemia cell line, fibroblast, and hippocampal neuron. Growth inhibitions were observed as ES potential was increased. In NIH 3T3, ES induced the expression of the inward rectifier K+ channel (Kir) Kv3.1. Previous studies supported that the overexpression of Kir induced the cell cycle arrest in G0/G1. However, K562 experiment was different in that Kv3.1 was not expressed though the growth inhibition was detected. Interestingly, mild PKC activator decursin was effective to diminish the cell growth inhibition induced by electrical stimulation. Activated PKC isozymes induced by decursin reduces currents via Kv3.1 so that cell proliferation may recovered in NIH 3T3, but mechanism in K562 seemed to be different. Decursin was also effective on hippocampal neuron under the ES condition. Our results indicate that decursin be used as an anticonvulsant drug to protect neurons in the same mechanism.