원문정보
초록
영어
Recombinant human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) were expressed from transgenic rice cell suspension cultures using RAmy3D promoter system.1) Since the cell wall of plant cells can be a barrier against protein secretion, the target protein, hCTLA4Ig, can be caught inside cell wall or remained in intracellular space.2) Nonionic surfactant, Triton X-100, a surfactant, has been proposed as a suitable agent for the permeabilization of plant cells.3) To promote the secretion of hCTLA4Ig caught in cytoplasm and cell walls, Triton X-100 were treated at 0.01, 0.05, 0.1% (v/v). Application of 0.01% Triton X-100 into transgenic rice cell cultures shows the positive effect on the secretion of hCTLA4Ig. With improved permeability, production level of hCTLA4Ig was also increased. Therefore, the production and permeabilization of hCTLA4Ig could be enhanced by the optimal addition of Triton X-100 in transgenic rice cell cultures.