원문정보
초록
영어
A global regulatory protein, AfsS-p from S. peucetius was identified by genomic search. Like other homologues, AfsS-p was a small protein consisting of 65 aminoacid, had the repeated conserved sequence, GDHHSPXPP(X is A or V) and enhanced antibiotic production; doxorubicin in S. peucetius and actinorhodin and undecylprodigiosin production in both S. lividans and S. coelicolor. Moreover, we could fine a putative AfsR binding site whose repeated sequence is CGTTCATCG in the promoter region of afsS-p. In order to consider whether co-expression of components from the AfsK-AfsR-AfsR system can lead more antibiotic production than the expression of one component, we made the transformants into which one or two components of this system were overexpressed. Comparing the level of antibiotic production from AfsK, AfsR, AfsS and its combined strains shows that co-expressing components of the AfsK-AfsR-AfsS system does not have the synergistic effect to overproduce more antibiotics than single expression.