원문정보
초록
영어
To screen plant growth promoting rhizobacteria (PGPR) to be able to colonize on plant roots and influence on plant growth, a specific promoter-capture vector was constructed and evaluated. The promoter capture vector (pCM-gfp) comprised a promoterless chloramphenicol resistance (acetyl transferase) gene for promoter screening and a green fluorescent protein (GFP) gene for the measurement of promoter activity. Metagenomic DNA was extracted from rhizosphere soil samples and bacteria from plants including rice, barley, wheat and rye. Metagenomic DNA was digested partially by Sau3AI and metagenomic libraries were constructed using the constructed pCM-gfp vector. Clones growing on LB agar with chloramphenicol (30mg/L) were screened and their promoter activities were evaluated using inducers such as plant extracts, naringenin, quercetin, or indole-3-acetic acid (IAA), which are known to influence on plant growth and induce
microbial gene expression. In addition to promoter capture screening, we screened IAA and 1-aminocyclopropane-1-carboxylate (ACC) deaminase producing microorganisms using a HPLC analysis and a PCR approach. More discussion will be done in detail.