원문정보
초록
영어
Newcastle disease virus (NDV) affects both domestic and wild bird and has become a major problem in many countries where poultry are reared. It damages the poultry industry seriously. Specific pathogen free (SPF) eggs have been used in the production of NDV-vaccine. However, wastes from eggs have caused serious environmental pollution. If NDV-vaccine is produced by mammalian cell culture, it can be produced in smaller space, with less labor. Although the virus can be grown in a various culture systems, its productivity is lower than the corresponding titer in embryonating eggs. To enhance virus productivity, it is essential to optimize environmental factors for the production of NDV. In this study, the optimal conditions to achieve the highest viral
titer are analyzed by some specific trials with MDBK cells and NDV. The stability of NDV at various temperaturesand sensitivity to freezing and thawing are analyzed for NDV handling and storage. The optimal incubation time after NDV inoculation was examined. The growth kinetics of MDBK for NDV production was investigated in different sugar composition media. The pH change of MDBK cells in various carbon source media was also examined.