earticle

영어

Swollenin, a fungal protein, is presumed to disrupt hydrogen bonding between cell wall polysaccharides. Despite of the disruption of hydrogen bonding, the swollenin is known not to possess the hydrolytic activity against cellulose. Therefore, the swollenin is thought to play some roles other than hydrolysis of cellulose when the fungi attack the lignocellulose which is the main body of plant biomass. In this study, the gene encoding swollenin was cloned from Trichoderma reesei into Saccharomyces cerevisiae. The activity of the swollenin was then evaluated by quantifying how much the swollenin preparation assist the action of cellulose on the cellulose when the protein was incubated with cellulose and cellulase.