원문정보
초록
영어
Lipases are used as efficient biocatalysts in many industrial processes for food, detergent, fine chemical, and biodiesel. Recently, Candida antarctica lipase B (CalB) draws attention because it can be used for stereoselective transformations and polymer synthesis and the potential process for biodiesel production using lipases instead of chemical catalysts is under development. Since E. coli expression systems are extensively studied and have advantages of ease to use
and low cost for screening of gene libraries as compared with the other expression systems, E. coli strains, Rosettagami with enhanced disulfide bond formation, Novablue, and DH5α, were exploited in this study. CalB was cloned into the plasmid pCold, and the constructed pCold/CalB was used to transform the E. coli strains. In addition, the host cells having the pCold construct were transformed with chaperone plasmids containing groES/groEL, groES/groEL/tig,
tig, dnaK/dnaJ/grpE, and dnaK/dnaJ/grpE/groES/groEL for coexpression of CalB and chaperones. The colonies expressing functional lipase were selected for each E. coli strain, and the CalB expression levels were examined.