earticle

영어

For screening of Q10-producing photosynthetic bacteria (PSB), we took samples from three different sites: silt at Nakdonggang, aquaculture farm and PSB production plant. Three PSB were isolated and found to be Rhodobacter sphaeroids, Rhodobacter capsulatus and Rhodopseudomonas palustris, respectively by the 16s-rDNA sequence analysis. The specific growth rate of R. sphaeroids cultivated on the basal medium was calculated to be 0.13 h-1. The
result of experiment in 1 L bioreactor showed that dissolve oxygen in the broth was depleted within 6 h, pH increased and ORP decreased finally. From the HPLC analysis, it was found that R. sphaeroids had the highest Q10 content, 59.2 mg/L among the three PSB.

한국어

Coenzyme Q10을 생산하는 광합성세균을 분리하기 위해 부산 낙동강하구, 양어장, 광합성세균 생산 공장에서 각각 광합성 균을 샘플링하여 총 3종을 분리하였고, 16s-rDNA sequencing을 통해 동정한 결과 Rhodobacter sphaeroids, Rhodobacter capsulatus 및 Rhodopseudomonas palustris로 밝혀졌다. 기초배지에서 배양한 R.sphaeroids균의 비증식속도는 0.13 h-1이였고, 1L 반응기에서의 배양한 결과 반응6시간 이내에 용존산소는 소모되었고, 최종 pH는 증가하고 ORP는 감소함을 알 수 있었다. HPLC 분석결과 R. sphaeroids균이 다른 균에 비해 가장 높은 Q10값(59.2mg/L)을 가짐을 알 수 있었다.