원문정보
초록
영어
Ntirogen source dependent growth and antibiotics production regulator (NdgR) was initially found from S. peucetius by DNA affinity capture assay method. NdgR was well conserved not only through the completely sequenced Streptomcyes species such as S. coelicolor and S. avermitilis, but also through many other bacteria such as Mycobacteria and Corynebacteria. Furthermore, it is quite different from known IclR-type regulators, which are known to be involved in glycerol operon, acetate production and sporulationspecific cell division and morphogenesis. In S. coelicolor, ndgR deletion mutant showed overall late growth and promoted actinorhodin (ACT) production in minimal media with various amino acids, even where wild type strain could not produce ACT. According to RT-PCR results, it was observed that NdgR affects glutamine/glutamate and proline. Moreover, surprisingly, it controls S. coelicolor quorum sensing system such as scbR/scbA by binding to its promoter region and autoregulate itself.