원문정보
초록
영어
Ghost vaccine production from E.coli / S.iniae 4 different type antigens -enolase, GAPDH, sagA and piaA was maximized α by the optimization of condition parameters such as glucose concentration, agitation speed, aeration, ghost efficiency, and induction temperature point. The glucose concentrations were adjusted to 0g/L, 1g/L, 2g/L and 3g/L in LB broth containing 50㎕/ml ampicillin for carbon source optimization. The highest cell densities in the cultures were 1.87g/l, 1.63g/l, 1.51g/l and 1.60g/l for E.coli / S.iniae antigens α-enolase, GAPDH, sagA and piaA with 1g/l of glucose, respectively. The effect of agitation speed was evaluated ranging
from 100 to 400 rpm. The maximum production of cell density was obtained at the agitation speed of 300 rpm with the concentration of 2.5g/l, 2.18g/l, 1.89g/l and 1.95g/l for E.coli / S.iniae antigens α-enolase, GAPDH, sagA and piaA, respectively. The effect of aeration rate on cell density was examined with the different aeration rates of 0.5, 1.0, 1.5 vvm at the agitation speed of 300rpm. Maximum production of cell density was obtained at the aeration rate of 1.0vvm with the concentration of 2.5g/l, 2.18g/l, 1.89g/l and 1.95g/l for E.coli / S.iniae antigens α-enolase, GAPDH, sagA and piaA, respectively. The induction temperature point was determined in three exponential phases, initial exponential phase(OD=1.0), mid exponential phase(OD=2.0), final exponential phase(OD=3.0). At the mid exponential phase(OD=2.0) and the ghost efficiencies was 99.92%. 99.67% and 97.72%, respectively. Therefore the optimal condition parameters of the
batch cultures were as follows : glucose concentration of 1g/l, agitation of 300rpm, aeration of 1vvm and induction temperature point of O.D : 2.0. Ghost induction with Streptococcus iniae antigens was analyzed by SDS-PAGE and Western blot