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논문검색

Free Radical Scavenging Activity of Zooshikella sp. CH-34 Extracts by Electron Spin Resonance Spectrometry

초록

영어

A bacterial strain producing a high concentration of red pigment isolated from sediment that had been collected East China Sea, which was recorded as CH-34. It was identified as Zooshikella sp.. They produced pigments with maximum absorption at 540 nm, which indicated the presence of prodigiosin1). The radical scavenging activities of the extracts from
CH-34 cell and culture broth according to extraction solvents and fractions on hydroxyl, alkyl and DPPH radical were investigated using a ESR spectrophotometer and compared with the ESR signal intensity. The extracts exhibited strong scavenging activity on hydroxyl, alkyl and DPPH
radical, and the activity increased with increment of concentration of the extracts. The electron donating abilities (EDAs) of ZC acetone extract (ZCEA) and ZC ether soluble fraction (ZCFE) were 95.74% and 94.51% at 0.5㎎/㎖ respectively. The hydroxyl radical scavenging ability of the ZC hot water extract (ZCE70W) was highest(59.08% at 1㎎/㎖), and alkyl radical scavenging ability was highest in the ZC water extract (84.05% at 1㎎/㎖). From these results, ZC extracts and fractions had strong antioxidant activities.

저자정보

  • Ju-Sang, Kim Dept. of Aquatic Life Medicine, Cheju National University
  • Hyoun-shik, Mun Dept. of Aquatic Life Medicine, Cheju National University
  • Moon-Soo, Heo Dept. of Aquatic Life Medicine, Cheju National University

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