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논문검색

Optimization for production of cellulases by Psychrobacter aquimaris LBH-10 isolated from sea-water

초록

영어

More than three hundreds microorganisms to hydrolysis carboxylmethylcellulsoe (CMC) were isolated from the seashore of the Kyungsang province in Korea. Among them, twelve microorganisms showed relatively higher activity of cellulase. Activity expressed as unit of cellulase by twelve microorganisms ranged from 35 to 110 unit with the liquid medium containing 2.0%(w/v) glucose, 0.25% yeast extract, 0.5% K2HPO4, 0.1% NaCl, 0.02% MgSO4·7H2O and
0.06% (NH4)2SO4 at 30°C for 72 hr under aerobic conditions. The best carbon source and nitrogen sources for the production of cellulase by P.aquimaris LBH-10 were found to be rice bran and peptone. CMC, Filter paper, Avicel and pNPG activities of the cellulase produced by P.aquimaris LBH-10 with 2.0%(w/v) rice bran and 0.5%(w/v) peptone were 929.97, 109.04, 94.37 and 4.38 unit for 72hr respectively.

저자정보

  • Hye-Jin Kim Department of Medical Bioscience, Graduate School of Dong-A University
  • Bo-Hwa Lee Division of Applied Biotechnology
  • Bo-Kyung Kim Division of Applied Biotechnology
  • You-Jung Lee Department of Medical Bioscience, Graduate School of Dong-A University
  • Gongyuan Wei BK21 Bio-Silver Group, Dong-A University,
  • Chung-Han Chung Division of Applied Biotechnology, BK21 Bio-Silver Group, Dong-A University
  • Jin-Woo Lee Division of Applied Biotechnology, BK21 Bio-Silver Group, Dong-A University

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