원문정보
초록
영어
A putative EHase (EEH1) was identified by analyzing open reading frames of Erythrobacter litoralis HTCC2594, the phylogenetic analysis of the putative gene showed that eeh1 was similar to microsomal EHase. The eeh1 gene was cloned and the recombinant (rEEH1) was purified. Kinetic resolution of SO and ECH using the purified EEH1 of E. litoralis HTCC2594 was performed. Optically active (S)-SO with an enantiomer excess (ee) higher than 97% was obtained from its racemate with a yield of 10% (theoretical 50%). It was not high, compared to the activities of the pervious report. On the other hand, the hydrolysis of the (R)- ECH proceeds at a much higher rate than the hydrolysis of the (S)- ECH. Enantiopure (S)-ECH could be obtained with an ee
value higher than 97% with a yield of 32%. Enantiopure ECH is a valuable epoxide intermediate for preparing optically active pharmaceuticals1). EEH1 from E. litoralis HTCC 2594 can be applied to bioprocess for the production of optically pure epoxides in the pharmaceutical industry. [This work supported by KORDI in house program (PE97803) and the Marine and Extreme Genome Research Centre Program, Ministry of Marine Affairs and Fisheries, Republic of Korea.]