원문정보
초록
영어
Bacillus subtilis subsp. subtilis A-53 was isolated from the seashore of the Kyungsang province in Korea.1) The highest production of cellulase was obtained when concentrations of rice hull and yeast extract were 5.0% (w/v) and 0.0% (w/v). Optimal initial pH of the medium and temperature were 6.8 and 37℃. Under these conditions, optimal agitation speed and aeration rate in a 7L bioreactor were found to be 400 rpm and 1.0 vvm.2) The gene coding cellulase
in B. subtilis subsp. subtilis A-53 was isolated and cloned. The deduced amino acid sequence of the cellulase showed high identity to cellulase from other Bacillus species. E. coli JM109 was used as a host strain for cloning and maintenance of plasmid. The production of cellulase by B. subtilis subsp. subtilis A-53 and its transformant from rice bran as a carbon source was 68.7(U/ml) and 868.8(U/ml),respectively.