원문정보
초록
영어
Sphingolipids are important structural components of eukaryotic membranes and are thought to provide rigidity to these structures. Along with this architectural role, sphingolipids and their precursor molecules are major participants in the regulation of cell proliferation and function.1) Ceramide is not only a core intermediate of sphingolipids but also an important modulator of many cellular events including apoptosis, cell cycle arrest, senescence, differentiation, and
stress responses. Though the effectiveness of ceramide is not understood fully, ceramide has become a widely used ingredient in cosmetics and pharmaceutical industries. Here we investigated the effect of three genes, Lac1, Lag1, and Sur2. In ceramide pathway, a very long chain fatty acyl-CoA is linked by and amide bond to DHS or PHS to generate dihydroceramide or phytoceramide. This step is catalyzed by Lac1 and Lag1. DHS is hydroxylated at C4 to yield PHS, which is the primary sphingoid base bound in most fungal and plant ceramide. This
reaction is catalyzed by Sur2. The genes were cloned on pYES2 vectors. S. cerevisiae was cultured in a batch fermentor and analyzed by HPLC using ELSD after cell lysis followed by solvent extraction.2)