원문정보
초록
영어
Recently, a genetically engineered succinic acid overproducing mutant, M. succiniciproducens LPK7, was developed based on full genome sequence. 2-DE and LC-MS/MS were used to analyze proteome of the mutant cells at the exponential and stationary phases. The results were compared with those of the wild type strain (MBEL55E) to elucidate the global physiological and metabolic changes responsible for succinic acid overproduction. Comparative proteomic
analyses between the MBEL55E and the mutant strain showed the apparent differences in 87 and 69 protein spots at the exponential and stationary phases, respectively. As the mutant cells grow, the expression levels of 58 proteins also changed. Several features, indispensable for further improving the succinic acid producers by rational metabolic engineering, will be described in detail. [This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (2005-01294). Further supports
by the LG Chem Chair Professorship, IBM SUR program, Microsoft, and by the KOSEF through the Center for Ultramicrochemical Process Systems are appreciated.].